牛腎由来株化細胞を用いた牛ウイルス性下痢ウイルスの
ウイルス分離法および抗体検査法

齋藤俊哉^1）†　　　　山口　修^2）　　　　深井克彦^1）

要　　　　　約 　従来，牛ウイルス性下痢ウイルス（BVDV）の培養細胞を用いた検査は，牛胎子筋肉（BFM）等を用いて，細胞病原性（CP）型は細胞変性効果（CPE）を指標に，CPEを示さない非細胞病原性（NCP）型は干渉法で実施されている．今回，牛腎由来株化（MDBK-SY）細胞が従来のCP型，NCP型ともにCPEを示す特性を利用したBVDV検査法を検討した．MDBK-SY細胞で野外材料4例から4株のBVDVを分離した．これら4株は，BFM細胞では，CP型1株，NCP型3株に分類された．また，野外血清155例と既知のBVDV・Nose株，No. 12株およびKS86-1NCP株による中和試験を，MDBK-SY細胞とBFM細胞で比較したところ，有意な相関が認められた．以上の成績から，MDBK-SY細胞のBVDV検査における有用性が確認された． ―キーワード：BVDV，CPE，MDBK-SY細胞． --------------------日獣会誌　56，717〜721（2003） 　 Antibody Tests and Isolation of the Cytopathogenic Bovine Viral Diarrhea Virus Toshiya SAITO＊†, Osamu YAMAGUCHI and Katsuhiko FUKAI ＊Tochigi Prefectural Kenou Animal Hygiene Service Center, 6-8 Hiraide-Kogyo-Danchi, Utsunomiya, 321-0905, Japan SUMMARY 　Antibody tests and isolation of the bovine diarrhea virus (BVDV) have been performed on primary bovine fetal muscular-cell cultures. The cytopathogenic effect (CPE) was the index in the case of cytopathogenic viruses. The interference method was employed in dealing with non-cytopathogenic types, which demonstrate no CPE. In this study, we made use of the characteristic of bovine kidney cell line (MDBK-SY) to be induced the CPE by both CP and NCP viruses and examined the possibility of a new BVDV assay method. This new assay was used in isolating 4 BVDVs from field specimens. Previous assays had identified one of these strains as CP BVDV and the other as NCP BVDV. Both previous assays and the new assay were used to determine the neutralization antibody titers to Nose, No. 12, and KS86-1 NCP in serum specimens. The neutralization antibody titers to those strains determined by the new assay correlated significantly with those determined by previous assays. These results suggest that the new assay established in this study is a useful system for BVDV investigation. ―Key words : bovine viral diarrhea virus, CPE, MDBK-SY cells. †　Correspondence to : Toshiya SAITO (Tochigi Prefectural Kenou Animal Hygiene Service Center) 6-8 Hiraide-Kogyo-Danchi, Utsunomiya, 321-0905, Japan TEL 028-689-1274 FAX 028-689-1279 ------------------J. Jpn. Vet. Med. Assoc., 56, 717〜721 (2003)