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要 約
食鳥処理場において，肉眼的にマレック病（MD）を疑って収集された33〜79日齢のブロイラー45例の肝臓および脾臓を病理組織学的に検索した．肉眼的には，肝臓および脾臓の2〜3倍に至る腫大がほとんどの例で認められ，多発性微小白色斑およびまれに大きな白色結節形成を伴っていた．病理組織学的に，9例（20％）はMDと診断されたが，35例（78％）では肝臓のグリソン鞘，脾臓の莢動脈周囲および白脾髄における紡錘形細胞増殖を特徴とする腫瘍様増殖性疾患が認められた．この増殖細胞は主として紡錘形あるいは多形性を示し，大小不同で豊富な好酸性細胞質を持っていた．トリ白血病ウイルスJ亜群（ALVhJ）のH5およびH7プライマーを用いたRThPCR法では，5例中4例でALVhJの特異的配列が増幅された．
―キーワード：トリ白血病ウイルスJ亜群，ブロイラー，マレック病，紡錘形細胞，腫瘍様増殖性疾患．
--------------------日獣会誌 54，127〜131（2001）
Spindle-cell Proliferative Disease in Broiler Chickens
Suspected of Marekﾕs Disease at Meat Inspection
Shigeaki TAKAMI＊, Masanobu GORYO†, Jun SASAKI, Takanori MAEJIMA,
Jouji HONDA, Hiroshi FURUKAWA, Kahori HONDA and Kosuke OKADA
＊Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan
SUMMARY
The livers and spleens of 45 broiler chickens (33 to 79 days old) suspected of Marekﾕs
disease (MD) at meat inspection were collected and examined histopathologically. In most cases, the organs
were enlarged from two to three times in size, and multiple, small, white areas of plaque or infrequent,
large, white nodules were observed. Although histopathologically only 9 birds (20%) were diagnosed as
MD, 35 birds (78%) demonstrated tumor-like spindle-cell proliferative lesions in the Glissonﾕs sheath
of the liver and in the white pulp and around the sheathed arteries of the spleen. The proliferating
cells were mainly spindle-shaped or pleomorphic, and variable in size with abundant eosinophilic cytoplasm.
Reverse transcriptase-polymerase chain reaction (RThPCR) based on primer H5 and H7 of the avian leukosis
virus strain J (ALVhJ) produced specific amplifications of ALVhJ in 4 of 5 cases.
―Key words : ALVhJ, broiler chicken, Marekﾕs disease, spindle-cell, tumor-like proliferative lesion.
† Correspondence to : Masanobu GORYO (Faculty of Agriculture, Iwate University) 3-18-8 Ueda, Morioka
020-8550, Japan
TEL 019-621-6217 FAX 019-621-6274
--------------------J. Jpn. Vet. Med. Assoc.、54, 127〜131 (2001) |
