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要 約
1982〜1998年の間にわが国の野外の病犬から，VeroあるいはB95a細胞を用いて分離された15株の犬ジステンパーウイルス（CDV）の生物学的および血清学的性状について検討し，同時にわが国のCDVワクチン株と比較した．鶏胎子線維芽細胞での増殖能，ポック形成能あるいはマウス脳内接種による神経病原性はワクチン株とVero細胞で分離・継代したいくつかの株に認められた．野外分離株9株とワクチン株の合計10株の交差中和試験の結果
，野外分離株間では大きな抗原性の差は認められなかったが，ワクチン株とは血清学的に差があることが明らかになった．しかしながら，ワクチン株は広い抗原性を持ち，その抗血清は野外分離各株に対して高い中和活性を示したことから，現行のワクチン株は有効であると考えられた．
―キーワード：生物学的性状，犬ジステンパーウイルス，犬，野外分離株，血清学的性状．
--------------------日獣会誌 109〜114（2001）
Biological and Serological Properties of Canine Distemper
Viruses Recently Isolated from Dogs in Japan
Motoko HATANO, Satoshi TAHARAGUCHI, Kayo IWAMOTO,
Katsuo MASUBUCHI, Teruaki KOKUBU, Osamu SASAKI and
Morimasa YAMANAKA†
＊Division of Veterinary Microbiology, Kyoto-Biken Laboratories,
24-16 Makishima-cho, Uji 611-0041, Japan
SUMMARY
Co-cultivation with Vero or B95a cell lines was used to study the biological and serological
properties of 15 strains of canine distemper virus (CDV) isolated from dogs in Japan from 1982 to 1998.
Those properties were then compared with those of the CDV vaccine strain used in Japan. The vaccine strain
and some of the field-isolates cultured in Vero cells demonstrated proliferation potency in chicken embryonic
fibroblast cell culture and pock-forming capacity and neurovirulence in suckling mice. Cross-neutralization
tests using 9 field- isolates, and the vaccine strain revealed no significant antigenic difference among
the field-isolates. The field-isolates were, however, somewhat different strain from the vaccine strain
in antigenicity. Since the antiserum to the vaccine dramatically neutralizes all the field-isolates,
the vaccine used currently in Japan can be considered effective against recently isolated CDV strains.
―Key words: biological properties, canine distemper virus, dog, field-isolates, serological properties.
† Correspondence to : Morimasa YAMANAKA (Division of Veterinary Microbiology, Kyoto-Biken Laboratories)
24-16 Makishima-cho, Uji 611-0041, Japan
TEL 0774-22-4518 FAX 0774-24-1407
--------------------J. Jpn. Vet. Med. Assoc.、54、109〜114 (2001) |
