鹿児島大学農学部(〒890-0065 鹿児島市郡元1-21-24) |
(1997年9月19日受付・1999年5月17日受理)
要 約
一般状態および生殖器所見に異常が認められない黒毛和種牛32頭およびホルスタイン種牛8頭計40頭に,発情時に人工授精を行い,発情後7日にウマ絨毛性性腺刺激ホルモン(eCG)500IU(13頭),ヒト絨毛性性腺刺激ホルモン(hCG)3,000IU(13頭)あるいは生理食塩液(14頭)を筋肉内投与して,直腸検査による卵巣の形態的変化および血中プロジェステロン(P4)濃度を調べた.受胎率はeCG群61.5%,hCG群46.2%,対照群35.7%であったが,各群間に有意差はなかった.発情後14日および21日の血中P4濃度は,eCG群およびhCG群が対照群と比べて高く,特に妊娠例では発情後7〜14日においてに有意に高かった(P<0.05).eCG群およびhCG群では,発情後14日の黄体直径が対照群より大きく,hCG群では副黄体が形成されて発情後40日においても存続していた.eCG群の卵巣には発情後14日に多数の小卵胞形成がみられたが,直径10mm以上の卵胞数は対照群と差がなかった.以上の成績から,発情後7日に低単位eCGを投与することによりhCG投与時と同様に黄体機能が賦活され,卵胞の過剰形成もなく,受胎率が向上する可能性が示唆された. ―キーワード:牛,eCG,hCG,黄体機能賦活. *現所属:長崎県北部農業共済組合(〒859-5151 平戸市紐差町798-1)
--------------------日獣会誌 52,635〜638(1999)
Luteotropic Effect of Equine Chorionic Gonadotropin(eCG) and Human Chorionic Gonadotropin (hCG) in CowsMasaki NAGANO*, Shunichi KAMIMURA,Kanji USHINOHAMA and Katsumi HAMANA
SUMMARY A smaller dose of equine or human chorionic gonadotropin, eCG (500 IU) or hCG (3,000 IU), was injected
on Day 7 of the estrous cycle in Japanese Black or Holstein cows; 13 cows for eCG, 13 cows for hCG and
14 cows for control and they were inseminated at estrus and examined for blood progesterone levels and
morphology of the ovaries by rectal palpation on Days 0, 5, 7, 14 and 21 of the estrous cycle. Two cows
of each group were examined for the diameter of corpus luteum by ultrasonography. The conception rates
of the eCG, hCG and control groups were 61.5%, 46.2%, and 35.7% respectively, while difference was not
significant. Blood progesterone levels were significantly higher in the pregnant cases treated with either
eCG or hCG on Days 14 and 21 as compared with non-pregnant controls (P<0.05), and small sized follicles
and accessory corpora lutea appeared on Day 14 in the eCG and hCG treated cows with significantly increased
diameter of corpora lutea. The results suggested that a smaller dose of eCG or hCG administered on Day
7 of the estrous cycle enhances the luteal function and consequently improves the pregnancy rate in cows. *Present address : Nagasaki-ken Hokubu A, M, A, A, 798-1 Himosashi-cho, Hirato-shi 859-5151, Japan
--------------------J. Jpn. Vet. Med. Assoc., 52, 635〜638 (1999) |